INTRODUCTION Analysis of Caffeine in Beverages by HPLC Essay

INTRODUCTION compendium of Caffeine in Beverages by HPLC - Essay ExampleIt is also addictive and the prevalence of caffeine in many drinks available today is a growing, serious health concern because it is a stimulant (Bidlingmeyer 385).The follow of caffeine present in soft drinks like Pepsi and Mountain Dew earth-closet be determined by the use high-performance liquid chromatography (HPLC). This is a process by which a assortment can be separated into its separate components or ingredients by the use of laboratory technique called as chromatography. In this process, the mixture to be separated is dissolved in a liquid, then it is passed or flushed through a stationary material (called as bed) and separated due to different rates of absorption of different materials. It is utilise to separate complex compounds with high precision utilise the right adsorbent materials and carrier fluid. A can (12 oz.) of Pepsi has about 38 mg (milligrams) in it while a Pepsi Max has about 69 mg . A can of Mountain Dew (both regular and diet) has approximately 54 mg of caffeine (Center for Science in the normal Interest para 2). Instant coffee has 65 mg, espresso has about 80 mg while Turkish coffee has one of the highest at about 160 mg. HPLC is simply an improved version of column chromatography that apply gravity to force separation but in HPLC, the process is made faster by using high pressure at up to 400 atmospheric